Preserving stem cells from extracted deciduous teeth

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) are a promising source of dental stem cells due to their high proliferation rate, multipotency, and ease of collection. This review highlights the classification and properties of dental stem cells, including SHED and their differentiation potential into odontogenic, osteogenic, neurogenic, and adipogenic cell types, as well as the conditions for the preservation of dental stem cells. SHED can be isolated from exfoliated deciduous teeth, particularly between ages 5 and 9, with the best results from teeth with healthy pulp. The cryopreservation process involves collecting, disinfecting, and expanding the stem cells, which are then stored for future regenerative medicine applications. Dental stem cell banking is becoming increasingly popular due to the non-invasive nature of the process and its cost-effectiveness compared to other sources of mesenchymal stem cells. However, challenges such as infection, lack of viable tissue, and a need for standardized manufacturing practices remain. Additionally, long-term studies are needed to confirm the efficacy of current cryopreservation techniques for future clinical use. The review concludes by emphasizing the potential of SHED in tissue regeneration and the importance of further research to optimize stem cell banking protocols and procedures.

How to cite this article:

Keskin M, Karaman K, Kasımoğlu Y. Preserving stem cells from extracted deciduous teeth. J Med Dent Invest 2025;6:e250063. https://doi.org/10.5577/jomdi.e250063