Comparison of hemostatic and acute histopathological effects of Algan Hemostatic Agent in experimental liver lobectomy model in rats

Abstract

Aim: Algan Hemostatic Agent (AHA) is a plant-based material effective in hemostasis. The purpose of this study was to compare the hemostatic efficacy and acute histopathological effects of liquid and powder forms of AHA in rat model of experimental liver lobectomy.

Methods: Twenty-four male Wistar albino rats, 10-12 weeks old, were randomly divided into three groups (n=8): control (physiologic saline), AHA-liquid, and AHA-powder. Following the resection of the outer one-third of the left hepatic lobe, physiological saline, liquid and powder, was administered to the injured area for twenty seconds. At the tenth minute, all animals were sacrificed through intra-abdominal bleeding. Liver tissues from all groups were excised for histopathological analysis.

Results: Bleeding control success rates of the first application results of AHA-liquid were higher than those of AHA-powder. Success rates of the second application of AHA liquid (66.6%) and AHA powder (50%) were substantially higher than those of the control group. The liver parenchyma was intact, and a dense fibrous tissue capsule was noted at the wound site of the liquid group. In powder group, late granulation tissue was observed along with moderate lymphocyte infiltration and some powder remnants.

Conclusion: The present study showed that herb-based AHA is an effective material for controlling blood loss in liver lobectomy model. It formed a stable clot without causing damage to liver tissue, especially when administered in liquid form. AHA could be a preferred and promising option for managing hemorrhage during crucial operations due to its biocompatibility and rapid efficacy.

How to cite this article:

Şener Akçora D, Mavuş D, Miko M, Gökçe K. Comparison of hemostatic and acute histopathological effects of Algan Hemostatic Agent in experimental liver lobectomy model in rats. J Med Dent Invest. 2024; 5: e240372.